Invitro Micropropogation of Nodal Explant of Hibiscus rosa-sinensis (L.) | Original Article
Hibiscus rosa-sinensis (L.) is a woody ornamental shrub belonging to Malvaceae family. The mass cultivation of hibiscus is hindered by Nematodes, bacterial and fungal infestation and is cultivated mostly by stem cutting method. The application of plant tissue technique recently has become a significant biotechnological tool in the multiplication of various plants that have a great economic importance in commercial plant production. Shoot initiation was established from nodal explants cultured on MS media supplemented with various combination of growth hormones at various ratios and concentrations. The HRS-9 showed best shoot elongation of 2.0 cm within 20 days and of 2.5 cm in 25 days at a hormones combination of 1.0 K (Kinetin) 0.2 IAA (Indole-3-acetic acid). At higher concentration of IAA with kinetin callus induction occurred.