Article Details

Diversity Analysis of Azotobacter Strains from Indian Agricultural Lands Using RFLP Technique | Original Article

Bhupendra Prasad*, Reena Antony, Aagosh Verma, in Career International Journal of Science and Technology | Science & Technology

ABSTRACT:

Azotobacter, a non-symbiotic nitrogen fixing bacteria plays a remarkable role in cycling of N2. Field trials have demonstrated that inoculation with Azotobacter has beneficial effects on plant yields. Present study was aimed to analyze the genetic diversity of Azotobacter sp. in soil of Madhya Pradesh, India. Twelve different strains of Azotobacter sp were isolated from rhizosphere of different agricultural fields and characterized through morphological, biochemical and genomic analysis. The phylogenic relation among these isolates was identified at molecular level by using 16S rDNA RFLP techniques. In RFLP analysis, similarity coefficient ranged from 0-0.35. On the basis of similarity pattern these twelve strains can be placed in four groups. Four isolates A6, A7, A8 A9 showed 100 similarity clearly indicating a very close relation with each other while a pair of isolates A1 A2 showed some distinct features which was evident from molecular evaluation in dendrogram.